REAL TIME PCR METHOD FOR PPV DIAGNOSTIC ON PLUMS AND APRICOT IN THE REPUBLIC OF NORTH MACEDONIA

Real-time PCR (Polymerase Chain Reaction) or qPCR is a method by which the amount of product can be determined in real-time, and very useful for investigating gene expression. The main advantages are that it provides fast high-throughput detection quantification target DNA sequences different matrices. lower time amplification facilitated simultaneous visualization newly formed amplicons. development application molecular methods pathogens has significantly changed diagnosis control plant diseases, various environmental samples, including hosts tissues, soil, water air. With real method, possible not only to identify detect presence absence pathogen, but also quantify present sample allowing quantitative assessment number pathogen sample. Detection accurate identification one most important strategies controlling diseases initiate preventive curative measures. Plum pox virus (PPV), agent Sharka, devastating infecting stone fruits. PPV mainly based on prevention, its quick reliable considered crucial this strategy. In study DAS-ELISA real-time were compared evaluating their potentialities limits large scale surveys. (Prunus domestica) apricot samples armeniaca) from several place included laboratory test analyzes, organs (phloem, buds, flowers, leaves fruits) parts them, periods year (spring, summer winter period 2017/20), symptoms comparison. Using tests, use universal set antibodies (BIOREBA), proved all examined especially collected summer, checking status early spring season. Testing found high concentrations viral antigens (OD 2.912-2.752, 30 min / 405 nm). Real show plot positive plums apricot.